Identification of phosphorylation sites in Hansenula polymorpha Pex14p by mass spectrometry
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Identification of phosphorylation sites in Hansenula polymorpha Pex14p by mass spectrometry
Pex14p is a peroxisomal membrane protein that is involved in both peroxisome biogenesis and selective peroxisome degradation. Previously, we showed that Hansenula polymorpha Pex14p was phosphorylated in vivo. In this study, we identified its phosphorylation site by mass spectrometry. Recombinant His-tagged Pex14p (H6-Pex14p) was overexpressed and purified from the yeast. The protein band corres...
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ژورنال
عنوان ژورنال: FEBS Open Bio
سال: 2012
ISSN: 2211-5463
DOI: 10.1016/j.fob.2012.11.001